^b BioMark/ISEP, School of Engineering, Polytechnic Institute of Porto, Porto, Portugal

^c Chemistry Institute, State University of São Paulo (UNESP), Araraquara, Brazil

A nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix assembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and differential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and morphological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid carboxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical performance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1 ng mL⁻¹ (r=0.995, p«0.01), with a very low limit of detection (0.006 ng mL⁻¹). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with excellent affinity to cTnT binding (K_D=7.3 10⁻¹³ mol L⁻¹). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications.