[2017-Vol.14-Issue 3]Enhance Stability and in vitro Cell Response to a Bioinspired Coating on Zr Alloy with Increasing Chitosan Content
Time: 2017-09-12 16:24  Click:350

Journal of Bionic Engineering

Volume 14, Issue 3, July 2017, Pages 459-467
Daniela Ionita1 , Maria Vardaki1 , Miruna S. Stan2 , Anca Dinischiotu2 , Ioana Demetrescu1,3
1. Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu, 011061, Bucharest, Romania
2. Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 SplaiulIndependentei, 050095, Bucharest, Romania
3. Academy of Romanian Scientist, SplaiulIndependentei 54, 050094, Bucharest, Romania


The aim of the present paper is to characterize bioinspired chitosan (CS) + hydroxyapatite (HA) coatings with various components ratio on a zirconium alloy with titanium. The coatings were characterized by FT-IR, SEM, hydrophilic/hydrophobic balance, adherence, roughness, electrochemical stability and in vitro cell response. Electrochemical tests, including potentiodynamic polarization curves and electrochemical impedance spectroscopy, were performed in normal saline physiological solution. Cell viability of MC3T3-E1 osteoblasts, lactate dehydrogenase, nitric oxide, and Reactive Oxygen Species (ROS) levels, as well as actin cytoskeleton morphology, were evaluated as biological in vitro tests. The results on in vitro cell response indicated good cell membrane integrity and viability for all samples, but an increased cell number, a decreased ROS level and a better cytoskeleton organization were noticed for the sample with a higher CS content. The coating with highest CS concentration indicated the best performance based on the experimental data. The highest hydrophilic character, highest resistance to corrosion and best biocompatibility as well recommend this coating for bioapplications in tissue engineering.


ZrTi alloy;
bioinspired coating;
cells response;
electrochemical stability

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