[2018-Vol.15-Issue 4]Preparation and Endothelialization of Multi-level Vessel-like Network in Enzymated Gelatin Scaffolds
Time: 2018-09-03 11:14  Click:133
Journal of Bionic Engineering
Volume 15, Issue 3, May 2018, Pages 673–681.
 
Guirong Dong1,2, Qin Lian3*, Laixia Yang1, Wei Mao3, Shiyang Liu3, Chao Xu1
1. School of Mechanical Engineering, Xi’an University of Science and Technology, Xi’an 710054, China
2. Faculty of Higher Vocational and Technical Education, Xi’an University of Technology, Xi’an 710082, China
3. State Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiao Tong University, Xi’an 710049, China
Abstract  Loss of function of large tissues is an urgent clinical problem. Although the artificial microfluidic network fabricated in large tis-sue-engineered constructs has great promise, it is still difficult to develop an efficient vessel-like design to meet the requirements of the biomimetic vascular network for tissue engineering applications. In this study, we used a facile approach to fabricate a branched and multi-level vessel-like network in a large muscle scaffolds by combining stereolithography (SL) technology and enzymatic crosslinking mechanism. The morphology of microchannel cross-sections was characterized using micro-computed tomography. The square cross-sections were gradually changed to a seamless circular microfluidic network, which is similar to the natural blood vessel. In the different micro-channels, the velocity greatly affected the attachment and spread of Human Umbilical Vein Endothelial Cell (HUVEC)-Green Fluorescent Protein (GFP). Our study demonstrated that the branched and multi-level microchannel network simulates biomimetic microenvironments to promote endothelialization. The gelatin scaffolds in the circular vessel-like networks will likely support myoblast and surrounding tissue for clinical use.
Key words: large-size scaffolds      vascular network      enzymatically gelatin hydrogel      endothelialization      muscle tissue engineering

Full text is available at:https://link.springer.com/article/10.1007/s42235-018-0055-3

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