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[2021-Vol.18-Issue 6]Critical Role of Silicon in Directing the Bio-inspired Mineralization of Gelatin in the Presence of Hydroxyapatite
Post: 2021-12-23 13:52  View:427

Journal of Bionic Engineering (2021) 18:1413–1429  https://doi.org/10.1007/s42235-021-00084-x

Critical Role of Silicon in Directing the Bio-inspired Mineralization of Gelatin in the Presence of Hydroxyapatite 

Ruijuan Yao1  · Yao Wang1  · Bo Zhang1  · Juan Liu1  · Nihui Zhang1  · Jing He1  · Guolong Meng1  · Bo Jiang1  · Shanling Wang2  · Fang Wu1

1 National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu 610064, China
2 Analytical and Testing Center, Sichuan University, Chengdu 601165, China 

Abstract  Signifcant progress has been made on understanding the critical role of organic components in directing the collagen mineralization. We hypothesize that the inorganic trace elements might also play important role in the mineralization of collagenous matrix. To this aim, we systematically compared the in-vitro biomineralization behaviors of gelatin, gelatin-HA and gelatin-SiHA electrospun membranes. The results indicated that the presence of Si ions played a striking infuence on the nucleation behaviors and mineralized structures. The gelatin-SiHA samples demonstrated more homogeneous nucleation within the gelatin fber and growth along the fber direction, in comparison with the heterogeneous nucleation and growth of spherulitic clusters on top of the nanofber surface, i.e. extrafbrillar mineralization. The likely shift of the nucleation mode to the intrafbrillar mineralization in the presence of Si ions led to good alignment of apatite c-axis with the long axis of the nanofber, resulting in a mineralization process and microstructure that were closer to those in natural bone. Cellular response analysis indicated that Si incorporation improved the MSC attachment and cytoskeleton organization. Such fndings might have important implication in both understanding the complex mechanisms involved in collagen mineralization and optimal designing of advanced bio-inspired materials with potential superior mechanical and biological properties. 

Keywords  Silicon · Hydroxyapatite · Biomineralization · Electrospinning · Nanofber · Gelatin

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SEM micrographs of gelatin, gelatin-HA and gelatin-SiHA electrospun fibers before (a–c) and after crosslinking (d–f). And their histogram of fiber diameters, respectively. The diameter of fibers was 174?±?39 nm, 187?±?51 nm and 190?±?66 nm for gelatin, gelatin-HA and gelatin-SiHA electrospun nanofibers before crosslinking and 287?±?56 nm, 302?±?81 nm and 270?±?80 nm for these samples after crosslinking respectively

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