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[2022-Vol.19-Issue 2]Impact of Hierarchical Architecture of Cryptotermes brevis Wing on the Modulation of Bacterial Adhesion
Post: 2022-04-07 20:54  View:149

Journal of Bionic Engineering (2022) 19:516–529  https://doi.org/10.1007/s42235-021-00148-y 

Impact of Hierarchical Architecture of Cryptotermes brevis Wing on the Modulation of Bacterial Adhesion 

Sanjeev Kumar Paikra1  · Sumit Mukherjee1  · Nibedita Nayak1  · Janmejaya Bag1  · Monalisa Mishra1

1 Neural Developmental Biology Laboratory, Department of Life Science, National Institute of Technology, 769008, Rourkela, Sundergarh, Odisha, India 

Abstract  The wing architecture is an inspiration to fabricate novel materials with exquisite properties. The current study characterizes the structure and biological function of a termite’s wing. The topography of the surface of the wing was studied by electron microscopy, and surface proflometer. The physicochemical property of the surface was analyzed by Fourier transform infrared spectroscopy, X-ray difraction spectroscopy, energy-dispersive X-ray spectroscopy, and gas chromatography–mass spectrometry analysis of the epicuticle content. Water Contact Angle measurement confrmed the hydrophobicity of the wing surface. When microorganisms come in contact with the surface of the wing, they adhere to the wing surface due to cell surface properties of their own and the surface chemistry of the wing. The current study reported the adhesion behavior of two bacterial species. The bactericidal activity of the wing was confrmed by counting the bacterial cell viability and examination under a confocal laser scanning microscope. Adhesion of bacteria was observed under the electron microscope. Bacterial oxidative stress, the topography of the wing, and the surface chemistry of the wing are the crucial factors that induce bactericidal activity. The nanostructure along with the chemical composition of the wing can be mimicked for the fabrication of novel material with antibacterial properties. 

Keywords  Bacterial adhesion · Bactericidal · Chitin · Nanostructure · Pseudomonas aeruginosa · Staphylococcus aureus

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Wing surface topography analysis: Photograph of termite a, SEM image of the CB wing at 2000X magnification b, Nanostructure at the wing dorsal surface 25000X c, Nanostructure at the wing ventral surface at 25000X d, the Surface profile of the CB wing indicating the height of the scales and nanostructures e, and Surface profile of the wing after chloroform treatment f. Nanostructure at the wing dorsal and ventral surface after chloroform treatment g and h, respectively

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