Journal of Bionic Engineering (2022) 19:483–496  https://doi.org/10.1007/s42235-021-00144-2 

Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing 

Jinzheng Zhang1  · Xiaoyu Lei1  · Jiajing Tang1  · Jie Chen1  · Qing Zhao1  · Wei Fang1  · Yinglong Zhang1  · Yubao Li1  · Yi Zuo1 

1 Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, People’s Republic of China

Abstract  Biomaterial-associated infection (BAI) is a kind of serious post-operative complication in orthopaedic surgery. Antibioticloaded bone cement shines a light on BAI prevention for convenient manipulation and complex flling. To this aim, we designed an antibacterial bone cement based on Nano-hydroxyapatite/Polyurethane (PUHA) loading with antibiotic Enoxacin (EN). The distinct shear-thinning behavior of the prepolymers was observed, indicating a good injectability. The PUHA bone cement possessed a suitable curing speed, and the addition of EN might slightly expedite the curing process and enhance the mechanical properties. The EN release profle indicated that the EN-loaded bone cement could reach the minimum inhibitory concentration in 2 h, and sustainedly released EN for almost 8 days, exhibiting an antibacterial delivery potential. Antibacterial test further confrmed the antibacterial ability of EN-loaded bone cement is in a dose-dependent manner. However, the osteogenic performance of drug-loaded bone cement with high dosage is not as good as antibacterial activity. When the EN concentration of antibacterial cement was lower than 32 μg?mL?1, the proliferation and osteogenic diferentiation of rat mesenchymal stem cells could be signifcantly promoted. Overall, this study verifed the potential of the EN-loaded PUHA bone cement in anti-infection and osteogenesis for bone repairing. 

Keywords  Antibacterial bone cement · Nano-hydroxyapatite/polyurethane · Enoxacin · Shear-thinning behavior · Osteogenesis

Graphical abstract of injectable EN-loaded bone cement based on PUHA promoting osteogenesis and inhibiting bacterial